9/13/2023 0 Comments Elisa assay vs western blotThese two antibodies are normally referred to as matched antibody pairs. This format requires two antibodies specific for different epitopes of the antigen. Sandwich ELISA (or sandwich immunoassay) is the most commonly used format. The method can also be used to detect specific antibodies in a serum sample by substituting the serum for the primary antibody.Įxplore indirect ELISA kits and reagents Sandwich ELISA As for direct ELISA assays, the antigen is immobilized to the surface of the multi-well plate. Indirect ELISA is a technique that uses a two-step process for detection, whereby a primary antibody specific for the antigen binds to the target, and a labeled secondary antibody against the host species of the primary antibody binds to the primary antibody for detection. ![]() In a direct ELISA, the antigen is immobilized to the surface of the multi-well plate and detected with an antibody specific for the antigen The antibody is directly conjugated to HRP or other detection molecules.
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